High Pressure Binary Gradient Pump Dual Piston For Sub-2µM Column UHPLC Separations
| Brand Name: | Echo |
| Model Number: | Echo |
| MOQ: | 1 Set |
| Price: | Contact Us for Pricing |
| Payment Terms: | T/T |
| Supply Ability: | 50+ Sets per Month |
High Pressure Binary Gradient Pump
,High Pressure binary gradient hplc
,Binary Gradient Pump with Dual Piston
This high-pressure binary gradient pump delivers precise, pulse-free solvent delivery across a 0.001 to 5.000 mL/min flow range at pressures scaling to 1300 bar. Engineered for sub-2µm column chemistries, it features a series dual-piston design with intelligent compressibility compensation, automatic prime, and integrated seal wash. Whether used as part of a UHPLC stack or standalone in a quaternary upgrade, this pump ensures retention time repeatability below 0.1% RSD and gradient accuracy of ±0.5%, session after session.
Gradient formation errors, pulsation, and seal leaks are the most common causes of HPLC data variability. A single piston pump with insufficient displacement creates baseline noise that mimics peaks; poor compressibility compensation shifts retention times as backpressure changes with column age. When your assay method requires a 20-minute gradient of 5–95% B and the actual delivered profile drifts 2%, critical impurity peaks may shift outside the specified retention time window, causing automatic batch rejection. These hidden pump problems eat analyst time and threaten regulatory findings.
Our binary pump uses two identical serial dual-piston pump heads operating in a primary/secondary arrangement. A dedicated microcontroller calculates solvent compressibility in real time from measured pressure, adjusting piston pre-compression stroke by stroke. The gradient is formed on the high-pressure side by a precision proportioning valve, achieving a dwell volume of just 35 µL. This enables extremely sharp gradient transitions. Inline vacuum degassing removes dissolved gases; an active seal wash prevents buffer crystal build-up. The pump transmits pressure, flow, and compressibility data to the CDS audit trail, making every stroke traceable.
| Parameter | Specification |
|---|---|
| Maximum Operating Pressure | 1300 bar (18,800 psi) |
| Flow Range | 0.001 – 5.000 mL/min in 0.001 increments |
| Flow Accuracy/Precision | ±0.1% / ≤0.05% RSD |
| Gradient Dwell Volume | 35 µL (standard) |
| Gradient Composition Accuracy | ±0.5% absolute |
| Degasser | 4-channel, integrated vacuum, <1 ppm O₂ |
| Seal Wash | Programmable, peristaltic pump |
| Materials | Titanium, PEEK, sapphire, ruby |
| Communication | Ethernet, RS-232, contact closure |
| Compliance | USP, EP, GLP, 21 CFR Part 11 ready |
- Sub-2 µm UHPLC method development
- Peptide mapping and biosimilar comparability
- Rapid LC-MS/MS bioanalytical assays
- High-resolution polymer and surfactant analysis
- Metabolomics fingerprinting
- Pharmaceutical forced degradation studies
When the pump initiates a gradient, the primary piston draws solvent A and B in sequence; the secondary piston compresses the mixed plug to system pressure before transferring it to the column. The pressure transducer continuously feeds back to the pump controller, which dynamically adjusts the pre-compression angle, guaranteeing a smooth, ripple-free flow even at 1300 bar. The online degasser uses a semi-permeable membrane under vacuum to strip dissolved oxygen and nitrogen, preventing outgassing in the detector cell.
Begin with your maximum required pressure: if you plan to use 1.7 µm particles at high flow rates, the 1300-bar model is essential. Select the pump head material based on mobile phase chemistry—titanium for standard reversed phase; PEEK for high-salt bio-separations. For method transfer from quaternary systems, note that binary gradient dwell volume is significantly lower; the run time must be adjusted. We provide a free gradient dwell volume calculator that predicts the new gradient profile so your method transfers smoothly.
A: Under typical usage with filtered mobile phase, seals last 12–18 months. With the active seal wash, lifetime often extends beyond 2 years. We recommend annual preventive maintenance.
A: Absolutely. The flow precision remains excellent down to 10 µL/min. For micro-flow LC-MS, a low-flow kit reduces system dispersion further.
A: Avoid neat hydrochloric acid and prolonged exposure to high-concentration sulfuric acid. Our chemical compatibility chart covers over 200 common HPLC solvents.
A: No, the degasser vacuum pump is fully integrated and electric-powered.